Monday, November 28, 2016

Of contaminated tissue culture

Assalamualaikum and Hi all...

Last September, we were having a lab session on tissue culture subject. We were required to do a seed-surface sterilization and in vitro culture of chilli seed where you had to establish an aseptic condition in laminar air flow, a place that looks like a cabinet or closet where you have to sterilize all the equipment to be used inside it even your hand that will be going to work in it ! And you have to sterilize all those things with alcohol such as ethanol. Swab the all those flask and beakers anddd your hand with the ethanol. Oh aseptic condition is a microorganisms-free environment means all those foreign things like bacteria fungi cannot infect the tissue culture work. Yeah I remember all those definitions of the tissue culture's terms! You know, we have to memorize and understand the terms (mostly new to me) that being used in tissue culture world and dua kali ditanya soalan definition of "habituation", dua kali jugak aku salah duhhh. This is an example when man (read:amni) didnt learn from mistake uhh. 

We have to be extra extra careful when working inside the laminar air flow cabinet. Make sure that you sterilize well, dont speak while work under the cabinet, seriously DO NOT SPEAK because you know your mouth contains lots of undesired microbes haha. If you take out your hand from the laminar air flow, you have to swab them back with ethanol bila nak masuk dalam tu balik. It needs lots lots lots of patience mann. If dont do it properly, the culture will be contaminated. And I had been given chance to do the culture of chilli seed andnn guess what? Both jar that I did were contaminated faceplam  WHATTTT. I dont know what are the causes but Dr said it is normal since it was my first time doing the tissue culture lab. But duhhh why Amni why ?? I messep up my group's lab result lol. Dr said it is normal to fail since that was my first doing this tissue culture lab.

When you are having extra creative group member, cara diorang menganjing aku buat lab tissue culture pastu contaminated, letak picture of my contaminated culture as whatsapp's group icon. Aku perati je korang ni heh

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