Of contaminated tissue culture

Assalamualaikum and Hi all...

Last September, we were having a lab session on tissue culture subject. We were required to do a seed-surface sterilization and in vitro culture of chilli seed where you had to establish an aseptic condition in laminar air flow, a place that looks like a cabinet or closet where you have to sterilize all the equipment to be used inside it even your hand that will be going to work in it ! And you have to sterilize all those things with alcohol such as ethanol. Swab the all those flask and beakers anddd your hand with the ethanol. Oh aseptic condition is a microorganisms-free environment means all those foreign things like bacteria fungi cannot infect the tissue culture work. Yeah I remember all those definitions of the tissue culture's terms! You know, we have to memorize and understand the terms (mostly new to me) that being used in tissue culture world and dua kali ditanya soalan definition of "habituation", dua kali jugak aku salah duhhh. This is an example when man (read:amni) didnt learn from mistake uhh. 

We have to be extra extra careful when working inside the laminar air flow cabinet. Make sure that you sterilize well, dont speak while work under the cabinet, seriously DO NOT SPEAK because you know your mouth contains lots of undesired microbes haha. If you take out your hand from the laminar air flow, you have to swab them back with ethanol bila nak masuk dalam tu balik. It needs lots lots lots of patience mann. If dont do it properly, the culture will be contaminated. And I had been given chance to do the culture of chilli seed andnn guess what? Both jar that I did were contaminated faceplam  WHATTTT. I dont know what are the causes but Dr said it is normal since it was my first time doing the tissue culture lab. But duhhh why Amni why ?? I messep up my group's lab result lol. Dr said it is normal to fail since that was my first doing this tissue culture lab.

When you are having extra creative group member, cara diorang menganjing aku buat lab tissue culture pastu contaminated, letak picture of my contaminated culture as whatsapp's group icon. Aku perati je korang ni heh

Piccolo pun Jadi Lah...

Weekend start on Friday, laying lazily on bed on Saturday, going out on Sunday. And class again on Monday *sigh* class... *sigh* Want to know why I sigh that much? Hahaha

Hari ni, kelas Practical Biology. Dan experiment hari ni adalah we are going to observe, observe, observe and examine slides of tissue. Kenapa banyak sangat observe tu? Because there are 16 slides of human and plant tissues that we had to observe, observe and observe over again. Yeah, enam belas selaid okkkayy and I was urghh poning kopalo den mengadap microscopes tu. And part yang paling aku tanak kawan adalah kitaorang kena lukis slide-slide tersebut. Yaaa, not all 16 but 7 only. Tujuh jerr pun, over bebeno mak cik ni.

Tapi tapi tapi, hakikat realiti yang aku tak tipu nya adalah drawing is toooo mainstream to me. Seriously, aku memang fail bab melukis ni. Bayangkan tujuh slides tisu-tisu tu aku kena lukis. Arghhh Im dying. Tanak tanak tanak ! Im a kind of clumsy and even my handwriting pun boleh kawan dengan ayam tengah cakar tanah haha. Apatah lagi nak melukis, skill tu mak aih kalau student art or architecture tengok geleng kepala tak habis. Even roommate aku pun gelak tengok lukisan aku. They were damn bad seriously.

Tapi ada jugak inisiatif nak practice but dah banyak trial aku buat, still damage haha. Agak-agaknya, kena masuk kelas belajar skill macam mana nak buat tangan aku lembut nanti lukisan jadi soft. And practice aku punya fokus tuh. Yeah, aku admit yang tangan ni keras semacam je haha buat line pun ketak-ketak je line tu aku tengok. And takleh fokus lama-lama nanti aku boring Haha. Because be a good biology student, pandai melukis is a must. My opinion laaa tambah kalau ambil laboratory subjek. Sebab korang akan lukis benda living things especially cells and tissues and many micro living things. But, part ni lah yang aku lemah sekali. Lain yang aku tengok kat slide, lain yang jadi bila aku lukis Haha.

So, ni nak kena keep trying and trying again or not my mark will be fly awayyyyyy. Haha. My Madam also cakap, buat leklok lukisan tu, if not, I have to give zero for your drawing. *sigh*

Errrr, ada orang nak tolong tak aku lukis slide slide ni semua? Bolehhhh takkk? *muka mintak simpati*

Yang ni seriously lupa apa nam dia Hahaha sorry

Tendon. Yang ni dah kali keberapa aku lukis still tak jadi wave dia tuh huh

This is the most beautiful for me. Areolar tissue.

This is Adipose tissue. Yeaa, a-di-po-se . sebut ikut ejaan dia yeee

Ephiteleum tissue in kidney tubule

Nervous tissue. Yang ni still buat aku nervous haha sebab tak tau nak lukis cemana. Arghhh tolonggg

6 of them. Satu lagi tak sempat amik gambar. Hehe. Bajet ambik gambar sebab aku tahu yang takkan siap punya semua lukisan tu dua jam dalam makmal Haha.

Aku rasa aku kena beli satu buku anatomy and practice to draw each of the diagram in the book. Practice make s perfect right? Malu jugak den kat classmate yang melukis woww sooo damn beautiful and neat *jeles jeles*

Wish to be Picasso but eventually jadi Piccolo. Hahaha Nasib nasibb

Hensem nya Piccolo nih, hijau kottt Wakaka

Assalamualaikum...